English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Deciphering the reading of the genetic code by near-cognate tRNA.

Blanchet, S., Cornu, D., Hatin, I., Grosjean, H., Bertin, P., & Namy, O. (2018). Deciphering the reading of the genetic code by near-cognate tRNA. Proceedings of the National Academy of Sciences of the United States of America, 115(12), 3018-3023. doi:10.1073/pnas.1715578115.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0000-FC20-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-70DB-8
Genre: Journal Article

Files

show Files
hide Files
:
2566973.pdf (Publisher version), 913KB
Name:
2566973.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2566973_Suppl.htm (Supplementary material), 181KB
Name:
2566973_Suppl.htm
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/xhtml+xml / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Blanchet, S.1, Author              
Cornu, D., Author
Hatin, I., Author
Grosjean, H., Author
Bertin, P., Author
Namy, O., Author
Affiliations:
1Department of Physical Biochemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_578598              

Content

show
hide
Free keywords: translation fidelity; stop codon readthrough; tRNA modification; genetic code; near-cognate tRNA
 Abstract: Some codons of the genetic code can be read not only by cognate, but also by near-cognate tRNAs. This flexibility is thought to be conferred mainly by a mismatch between the third base of the codon and the first of the anticodon (the so-called "wobble" position). However, this simplistic explanation underestimates the importance of nucleotide modifications in the decoding process. Using a system in which only near-cognate tRNAs can decode a specific codon, we investigated the role of six modifications of the anticodon, or adjacent nucleotides, of the tRNAs specific for Tyr, Gln, Lys, Trp, Cys, and Arg in Saccharomyces cerevisiae. Modifications almost systematically rendered these tRNAs able to act as near-cognate tRNAs at stop codons, even though they involve noncanonical base pairs, without markedly affecting their ability to decode cognate or near-cognate sense codons. These findings reveal an important effect of modifications to tRNA decoding with implications for understanding the flexibility of the genetic code.

Details

show
hide
Language(s): eng - English
 Dates: 2018-03-05
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1715578115
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 115 (12) Sequence Number: - Start / End Page: 3018 - 3023 Identifier: -