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  Fluorescence nanoscopy in cell biology

Sahl, S. J., Hell, S. W., & Jakobs, S. (2017). Fluorescence nanoscopy in cell biology. Nature Reviews Molecular Cell Biology, 18(11), 685-701. doi:10.1038/nrm.2017.71.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-FD3B-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-3F0A-F
Genre: Journal Article

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NatRevMolCellBiol_18_2017_685.pdf (Any fulltext), 14MB
 
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 Creators:
Sahl, Steffen J., Author
Hell, Stefan W.1, Author              
Jakobs, Stefan, Author
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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Free keywords: Cellular imaging; Fluorescence imaging; Super-resolution microscopy
 Abstract: Fluorescence nanoscopy uniquely combines minimally invasive optical access to the internal nanoscale structure and dynamics of cells and tissues with molecular detection specificity. While the basic physical principles of 'super-resolution' imaging were discovered in the 1990s, with initial experimental demonstrations following in 2000, the broad application of super-resolution imaging to address cell-biological questions has only more recently emerged. Nanoscopy approaches have begun to facilitate discoveries in cell biology and to add new knowledge. One current direction for method improvement is the ambition to quantitatively account for each molecule under investigation and assess true molecular colocalization patterns via multi-colour analyses. In pursuing this goal, the labelling of individual molecules to enable their visualization has emerged as a central challenge. Extending nanoscale imaging into (sliced) tissue and whole-animal contexts is a further goal. In this Review we describe the successes to date and discuss current obstacles and possibilities for further development.

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Language(s): eng - English
 Dates: 2017-09-062017-11-01
 Publication Status: Published in print
 Pages: 17
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 Table of Contents: -
 Rev. Method: Peer
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Title: Nature Reviews Molecular Cell Biology
Source Genre: Journal
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Publ. Info: London, UK : Nature Pub. Group
Pages: - Volume / Issue: 18 (11) Sequence Number: - Start / End Page: 685 - 701 Identifier: ISSN: 1471-0072
CoNE: https://pure.mpg.de/cone/journals/resource/110985821000941