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  Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues.

Lukinavicius, G., Mitronova, G., Schnorrenberg, S., Butkevich, A., Barthel, H., Belov, V. N., et al. (2018). Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues. Chemical Science, 9(13), 3324-3334. doi:10.1039/C7SC05334G.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-FD55-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-881E-3
Genre: Journal Article

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Lukinavicius, G.1, Author              
Mitronova, G.2, Author              
Schnorrenberg, S.2, Author              
Butkevich, A.2, Author              
Barthel, H.2, Author              
Belov, V. N.2, Author              
Hell, S. W.2, Author              
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              
2Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents – taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly (Drosophila melanogaster) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae.

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Language(s): eng - English
 Dates: 2018-02-262018-04-07
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1039/C7SC05334G
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Title: Chemical Science
Source Genre: Journal
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Pages: - Volume / Issue: 9 (13) Sequence Number: - Start / End Page: 3324 - 3334 Identifier: -