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  Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging

Gregor, C., Gwosch, K. C., Sahl, S. J., & Hell, S. W. (2018). Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proceedings of the National Academy of Sciences of the United States of America, 115(5), 962-967. doi:10.1073/pnas.1715946115.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-FD84-E Version Permalink: http://hdl.handle.net/21.11116/0000-0000-FD85-D
Genre: Journal Article

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 Creators:
Gregor, Carola, Author
Gwosch, Klaus C., Author
Sahl, Steffen J., Author
Hell, Stefan W.1, Author              
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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Free keywords: bioluminescence; luciferase; antibiotics; bacteria; microscopy
 Abstract: Bioluminescence imaging of single cells is often complicated by the requirement of exogenous luciferins that can be poorly cell-permeable or produce high background signal. Bacterial bioluminescence is unique in that it uses reduced flavin mononucleotide as a luciferin, which is abundant in all cells, making this system purely genetically encodable by the lux operon. Unfortunately, the use of bacterial bioluminescence has been limited by its low brightness compared with other luciferases. Here, we report the generation of an improved lux operon named ilux with an approximately sevenfold increased brightness when expressed in Escherichia coli; ilux can be used to image single E. coli cells with enhanced spatiotemporal resolution over several days. In addition, since only metabolically active cells produce bioluminescent signal, we show that ilux can be used to observe the effect of different antibiotics on cell viability on the single-cell level.

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Language(s): eng - English
 Dates: 2017-09-122018-01-16
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Method: Internal
 Identifiers: DOI: 10.1073/pnas.1715946115
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proceedings of the National Academy of Sciences of the USA
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 115 (5) Sequence Number: - Start / End Page: 962 - 967 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230