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  SRpHi ratiometric pH biosensors for super-resolution microscopy

Richardson, D. S., Gregor, C., Winter, F. R., Urban, N. T., Sahl, S. J., Willig, K. I., et al. (2017). SRpHi ratiometric pH biosensors for super-resolution microscopy. Nature Communications, 8: 577, pp. 1-9. doi:10.1038/s41467-017-00606-4.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-FE1A-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-FE1B-5
Genre: Journal Article

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NatCommun_8_2017_e577.pdf (Any fulltext), 2MB
 
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 Creators:
Richardson, Douglas S., Author
Gregor, Carola, Author
Winter, Franziska R., Author
Urban, Nicolai T., Author
Sahl, Steffen J., Author
Willig, Katrin I., Author
Hell, Stefan W.1, Author              
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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Free keywords: Biosensors; Endocytosis; Fluorescence imaging; Super-resolution microscopy
 Abstract: Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. One key application of pH probes has been to monitor the acidification of vesicles during endocytosis, an essential function that aids in cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250 nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy.

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Language(s): eng - English
 Dates: 2015-11-152017-07-072017-09-182017
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-017-00606-4
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 8 Sequence Number: 577 Start / End Page: 1 - 9 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723