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  Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores.

Kajtez, J., Solomatina, A., Novak, M., Polak, B., Vukušić, K., Rüdiger, J., et al. (2016). Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores. Nature Communications, 7: 10298.

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Kajtez, Janko1, Author           
Solomatina, Anastasia1, Author           
Novak, Maja, Author
Polak, Bruno1, Author           
Vukušić, Kruno, Author
Rüdiger, Jonas1, Author           
Cojoc, Gheorghe2, Author           
Milas, Ana3, Author
Šestak, Ivana Šumanovac1, Author           
Risteski, Patrik, Author
Tavano, Federica1, Author           
Klemm, Anna2, Author           
Roscioli, Emanuele, Author
Welburn, Julie, Author
Cimini, Daniela, Author
Glunčić, Matko, Author
Pavin, Nenad2, Author           
Tolić, Iva M2, Author           
Affiliations:
1Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              
2External Organizations, ou_persistent22              
3Max Planck Society, ou_persistent13              

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 Abstract: During metaphase, forces on kinetochores are exerted by k-fibres, bundles of microtubules that end at the kinetochore. Interestingly, non-kinetochore microtubules have been observed between sister kinetochores, but their function is unknown. Here we show by laser-cutting of a k-fibre in HeLa and PtK1 cells that a bundle of non-kinetochore microtubules, which we term 'bridging fibre', bridges sister k-fibres and balances the interkinetochore tension. We found PRC1 and EB3 in the bridging fibre, suggesting that it consists of antiparallel dynamic microtubules. By using a theoretical model that includes a bridging fibre, we show that the forces at the pole and at the kinetochore depend on the bridging fibre thickness. Moreover, our theory and experiments show larger relaxation of the interkinetochore distance for cuts closer to kinetochores. We conclude that the bridging fibre, by linking sister k-fibres, withstands the tension between sister kinetochores and enables the spindle to obtain a curved shape.

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 Dates: 2016
 Publication Status: Issued
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 Rev. Type: Internal
 Identifiers: eDoc: 732383
Other: 6413
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Title: Nature Communications
Source Genre: Journal
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Pages: - Volume / Issue: 7 Sequence Number: 10298 Start / End Page: - Identifier: -