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  Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro.

Woodruff, J., Wueseke, O., Viscardi, V., Mahamid, J., Ochoa, S. D., Bunkenborg, J., et al. (2015). Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Science (New York, N.Y.), 348(6236), 808-812.

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 Creators:
Woodruff, Jeffrey1, Author           
Wueseke, Oliver1, Author           
Viscardi, Valeria, Author
Mahamid, J.1, Author           
Ochoa, Stacy D, Author
Bunkenborg, Jakob, Author
Widlund, Per1, Author           
Pozniakovsky, Andrei I.1, Author           
Zanin, Esther, Author
Bahmanyar, Shirin, Author
Zinke, Andrea1, Author           
Hong, Sun Hae, Author
Decker, Markus1, Author           
Baumeister, W.2, Author
Andersen, Jens S, Author
Oegema, Karen1, Author           
Hyman, Anthony1, Author           
Affiliations:
1Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              
2Max Planck Society, ou_persistent13              

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 Abstract: The centrosome organizes microtubule arrays within animal cells and comprises two centrioles surrounded by an amorphous protein mass called the pericentriolar material (PCM). Despite the importance of centrosomes as microtubule-organizing centers, the mechanism and regulation of PCM assembly are not well understood. In Caenorhabditis elegans, PCM assembly requires the coiled-coil protein SPD-5. We found that recombinant SPD-5 could polymerize to form micrometer-sized porous networks in vitro. Network assembly was accelerated by two conserved regulators that control PCM assembly in vivo, Polo-like kinase-1 and SPD-2/Cep192. Only the assembled SPD-5 networks, and not unassembled SPD-5 protein, functioned as a scaffold for other PCM proteins. Thus, PCM size and binding capacity emerge from the regulated polymerization of one coiled-coil protein to form a porous network.

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 Dates: 2015
 Publication Status: Issued
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 Rev. Type: -
 Identifiers: eDoc: 718082
Other: 6225
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Title: Science (New York, N.Y.)
Source Genre: Journal
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Pages: - Volume / Issue: 348 (6236) Sequence Number: - Start / End Page: 808 - 812 Identifier: -