OpenSPIM: an open access light sheet microscopy platform

Pitrone, Peter Gabriel; Schindelin, Johannes; Stuyvenberg, Luke; Preibisch, Stephan W.; Weber, Michael; Eliceiri, Kevin W; Huisken, Jan; Tomancak, Pavel

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OpenSPIM: an open access light sheet microscopy platform

Pitrone, P. G., Schindelin, J., Stuyvenberg, L., Preibisch, S. W., Weber, M., Eliceiri, K. W., et al. (2013). OpenSPIM: an open access light sheet microscopy platform. Nature Methods, 10(7), 598-599.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0001-0718-D Version Permalink: https://hdl.handle.net/21.11116/0000-0001-0719-C

Genre: Journal Article

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Creators:
Pitrone, Peter Gabriel¹, Author
Schindelin, Johannes¹, Author
Stuyvenberg, Luke², Author
Preibisch, Stephan W.¹, Author
Weber, Michael¹, Author
Eliceiri, Kevin W, Author
Huisken, Jan¹, Author
Tomancak, Pavel¹, Author

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1Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692
2Max Planck Society, ou_persistent13

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Abstract: Light-sheet microscopy is revolutionizing biology by enabling live in toto imaging of entire embryos or organs with minimal phototoxicity1. We present an open hardware and software platform for constructing a customizable microscope for selective- plane illumination microscopy (SPIM). The OpenSPIM platform is shared with the scientific community through a public website (http://openspim.org/), making light-sheet microscopy more acces- sible so that it can be optimized for various applications.

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Dates: Date issued: 2013

Publication Status: Issued

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Identifiers: eDoc: 688524
Other: 5298

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Title: Nature Methods

Source Genre: Journal

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Pages: - Volume / Issue: 10 (7) Sequence Number: - Start / End Page: 598 - 599 Identifier: -