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  Slicing embryos gently with laser light sheets.

Huisken, J. (2012). Slicing embryos gently with laser light sheets. BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology, 34(5), 406-411.

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Huisken, Jan1, Author           
Affiliations:
1Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

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 Abstract: Light sheet microscopy is an easy to implement and extremely powerful alternative to established fluorescence imaging techniques such as laser scanning confocal, multi-photon and spinning disk microscopy. By illuminating the sample only with a thin slice of light, photo-bleaching is reduced to a minimum, making light sheet microscopy ideal for non-destructive imaging of fragile samples over extended periods of time. Millimeter-sized samples can be imaged rapidly with high resolution and high depth penetration. A large variety of instruments have been developed and optimized for a number of different samples: Bessel beams form thin light sheets for single cells, and selective plane illumination microscopy (SPIM) offers multi-view acquisition to image entire embryos with isotropic resolution. This review explains how light sheet microscopy involves a conceptually new microscope design and how it changes modern imaging in biology.

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 Dates: 2012
 Publication Status: Issued
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 Identifiers: eDoc: 645201
Other: 4768
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Title: BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
Source Genre: Journal
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Pages: - Volume / Issue: 34 (5) Sequence Number: - Start / End Page: 406 - 411 Identifier: -