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  Global analysis reveals SRp20- and SRp75-specific mRNPs in cycling and neural cells.

Änkö, M.-L., Morales, L., Henry, I., Beyer, A., & Neugebauer, K. M. (2010). Global analysis reveals SRp20- and SRp75-specific mRNPs in cycling and neural cells. Nature Structural & Molecular Biology, 17(8), 962-970.

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 Creators:
Änkö, Minna-Liisa1, Author           
Morales, Lucia1, Author           
Henry, Ian1, Author           
Beyer, Andreas2, Author
Neugebauer, Karla M.1, Author           
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1Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              
2Max Planck Society, ou_persistent13              

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 Abstract: Members of the SR protein family of RNA-binding proteins have numerous roles in mRNA metabolism, from transcription to translation. To understand how SR proteins coordinate gene regulation, comprehensive knowledge of endogenous mRNA targets is needed. Here we establish physiological expression of GFP-tagged SR proteins from stable transgenes. Using the GFP tag for immunopurification of mRNPs, mRNA targets of SRp20 and SRp75 were identified in cycling and neurally induced P19 cells. Genome-wide analysis showed that SRp20 and SRp75 associate with hundreds of distinct, functionally related groups of transcripts that change in response to neural differentiation. Knockdown of either SRp20 or SRp75 led to up- or downregulation of specific transcripts, including identified targets, and rescue by the GFP-tagged SR proteins proved their functionality. Thus, SR proteins contribute to the execution of gene-expression programs through their association with distinct endogenous mRNAs.

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 Dates: 2010
 Publication Status: Issued
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 Identifiers: eDoc: 546647
Other: 4203
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Title: Nature Structural & Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 17 (8) Sequence Number: - Start / End Page: 962 - 970 Identifier: -