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  Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid

De-Pietri-Tonelli, D., Calegari, F., Fei, J.-F., Nomura, T., Osumi, N., Heisenberg, C.-P., et al. (2006). Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid. BioTechniques, 41(6), 727-732.

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De-Pietri-Tonelli, Davide, Autor
Calegari, Federico1, Autor           
Fei, Ji-Feng1, Autor           
Nomura, Tadashi, Autor
Osumi, Noriko, Autor
Heisenberg, Carl-Philipp1, Autor           
Huttner, and Wieland B., Autor
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1Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

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 Zusammenfassung: The detection of microRNAs (miRNAs) at single-cell resolution is important for studying the role of these posttranscriptional regulators. Here, we use a dual-fluorescent green fluorescent protein (GFP)-reporter/monomeric red fluorescent protein (mRFP)-sensor (DFRS) plasmid, injected into zebrafish blastomeres or electroporated into defined tissues of mouse embryos in utero or ex utero, to monitor the dynamics of specific miRNAs in individual live cells. This approach reveals, for example, that in the developing mouse central nervous system, miR-124a is expressed not only in postmitotic neurons but also in neuronal progenitor cells. Collectively, our results demonstrate that acute administration of DFRS plasmids offers an alternative to previous in situ hybridization and transgenic approaches and allows the monitoring of miRNA appearance and disappearance in defined cell lineages during vertebrate development

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 Datum: 2006
 Publikationsstatus: Erschienen
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 Identifikatoren: eDoc: 311153
Anderer: 714
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Titel: BioTechniques
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 41 (6) Artikelnummer: - Start- / Endseite: 727 - 732 Identifikator: -