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  The Caenorhabditis elegans centrosomal protein SPD-2 is required for both pericentriolar material recruitment and centriole duplication.

Pelletier, L., Ozlu, N., Hannak, E., Cowan, C., Habermann, B., Ruer, M., et al. (2004). The Caenorhabditis elegans centrosomal protein SPD-2 is required for both pericentriolar material recruitment and centriole duplication. Current Biology, 14(10), 863-873.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-1271-B Version Permalink: http://hdl.handle.net/21.11116/0000-0001-1273-9
Genre: Journal Article

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 Creators:
Pelletier, Laurence1, Author              
Ozlu, Nurhan1, Author              
Hannak, Eva1, Author              
Cowan, Carrie1, Author              
Habermann, Bianca1, Author              
Ruer, Martine1, Author              
Muller-Reichert, Thomas1, Author              
Hyman, Anthony A1, Author              
Affiliations:
1Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

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 Abstract: BACKGROUND: The centrosome is composed of a centriole pair and pericentriolar material (PCM). By marking the site of PCM assembly, the centrioles define the number of centrosomes present in the cell. The PCM, in turn, is responsible for the microtubule (MT) nucleation activity of centrosomes. Therefore, in order to assemble a functional bipolar mitotic spindle, a cell needs to control both centriole duplication and PCM recruitment. To date, however, the molecular mechanisms that govern these two processes still remain poorly understood. RESULTS: Here we show that SPD-2 is a novel component of the C. elegans centrosome. SPD-2 localizes to the centriole throughout the cell cycle and accumulates on the PCM during mitosis. We show that SPD-2 requires SPD-5 for its accumulation on the PCM and that in the absence of SPD-2, centrosome assembly fails. We further show that centriole duplication is also defective in spd-2(RNAi) embryos, but not in spd-5(RNAi) embryos, where PCM recruitment is efficiently blocked. CONCLUSIONS: Taken together, our results suggest that SPD-2 may link PCM recruitment and centriole duplication in C. elegans. SPD-2 shares homology with a human centrosome protein, suggesting that this key component of the C. elegans centrosome is evolutionarily conserved.

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 Dates: 2004
 Publication Status: Published in print
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 Identifiers: eDoc: 229786
Other: 465
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Title: Current Biology
Source Genre: Journal
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Pages: - Volume / Issue: 14 (10) Sequence Number: - Start / End Page: 863 - 873 Identifier: -