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  Selective delivery of secretory cargo in golgi-derived carriers of nonepithelial cells

Rustom, A., Bajohrs, M., Kaether, C., Keller, P., Toomre, D., Corbeil, D., et al. (2002). Selective delivery of secretory cargo in golgi-derived carriers of nonepithelial cells. Traffic, 3(4), 279-288.

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Rustom, A., Author
Bajohrs, M., Author
Kaether, C., Author
Keller, P.1, Author           
Toomre, D., Author
Corbeil, D.1, Author           
Gerdes, H. H., Author
Affiliations:
1Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

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Free keywords: green fluorescent protein; multi-color imaging; polarized sorting; trans-Golgi network; transport carriers
 Abstract: In epithelial cells, soluble cargo proteins destined for basolateral or apical secretion are packaged into distinct trans-Golgi network-derived transport carriers. Similar carriers, termed basolateral- and apical-like, have been observed in nonepithelial cells using ectopically expressed membrane marker proteins. Whether these cells are capable of selectively packaging secretory proteins into distinct carriers is still an open question. Here, we have addressed this issue by analyzing the packaging and transport of secretory human chromogranin B fusion proteins using a green fluorescent protein-based high-resolution, dual-color imaging technique. We were able to show that these secretory markers were selectively packaged at the Golgi into tubular/vesicular-like transport carriers containing basolateral membrane markers, resulting in extensive cotransport. In contrast, deletion mutants of the human chromogranin B fusion proteins lacking an N-terminal loop structure were efficiently transported in both basolateral- and apical-like carriers, the latter displaying a spherical morphology. Similarly, in polarized epithelial cells, the human chromogranin B fusion protein was secreted basolaterally and the loop-deleted analogue into both the basolateral and apical medium. These findings suggest that nonepithelial cells, like their epithelial counterparts, possess a sorting machinery capable of selective packaging of secretory cargo into distinct types of carriers.

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Language(s): eng - English
 Dates: 2002-04
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 15403
ISI: 000174934100005
 Degree: -

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Title: Traffic
  Alternative Title : Traffic
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 3 (4) Sequence Number: - Start / End Page: 279 - 288 Identifier: ISSN: 1398-9219