ausblenden:
Schlagwörter:
proteomics; nanoelectrospray mass spectrometry; tandem mass spectrometry; protein sequencing
Zusammenfassung:
Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with trypsin and the recovered peptides can be sequenced de novo using triple quadrupole or hybrid quadrupole time-of-flight instruments equipped with a nanoelectrospray ion source. The peptide sequences determined provide useful information for identification of proteins by homology searching for cloning of the cognate genes by PCR based approaches.
