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  Translation initiation in bacterial polysomes through ribosome loading on a standby site on a highly translated mRNA.

Andreeva, I., Belardinelli, R., & Rodnina, M. V. (2018). Translation initiation in bacterial polysomes through ribosome loading on a standby site on a highly translated mRNA. Proceedings of the National Academy of Sciences of the United States of America, 115(17), 4411-4416. doi:10.1073/pnas.1718029115.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-1FC3-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-205C-2
Genre: Journal Article

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 Creators:
Andreeva, I.1, Author              
Belardinelli, R.1, Author              
Rodnina, M. V.1, Author              
Affiliations:
1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              

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Free keywords: global fitting; polysome formation; ribosome; translation initiation; translational efficiency
 Abstract: During translation, consecutive ribosomes load on an mRNA and form a polysome. The first ribosome binds to a single-stranded mRNA region and moves toward the start codon, unwinding potential mRNA structures on the way. In contrast, the following ribosomes can dock at the start codon only when the first ribosome has vacated the initiation site. Here we show that loading of the second ribosome on a natural 38-nt-long 5' untranslated region of lpp mRNA, which codes for the outer membrane lipoprotein from Escherichia coli, takes place before the leading ribosome has moved away from the start codon. The rapid formation of this standby complex depends on the presence of ribosomal proteins S1/S2 in the leading ribosome. The early recruitment of the second ribosome to the standby site before translation by the leading ribosome and the tight coupling between translation elongation by the first ribosome and the accommodation of the second ribosome can contribute to high translational efficiency of the lpp mRNA.

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Language(s): eng - English
 Dates: 2018-04-092018-04-24
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1718029115
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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 115 (17) Sequence Number: - Start / End Page: 4411 - 4416 Identifier: -