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  Generation of dense statistical connectomes from sparse morphological data

Egger, R., Dercksen, V. J., Udvary, D., Hege, H.-C., & Oberlaender, M. (2014). Generation of dense statistical connectomes from sparse morphological data. Frontiers in Neuroanatomy, 8: 129, pp. 1-18. doi:10.3389/fnana.2014.00129.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-2236-C Version Permalink: http://hdl.handle.net/21.11116/0000-0004-4276-D
Genre: Journal Article

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 Creators:
Egger, Robert1, 2, Author              
Dercksen , Vincent J., Author
Udvary, Daniel1, 2, Author              
Hege, Hans-Christian, Author
Oberlaender, Marcel1, 2, Author              
Affiliations:
1Former Research Group Computational Neuroanatomy, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_2528698              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              

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 Abstract: Sensory-evoked signal flow, at cellular and network levels, is primarily determined by the synaptic wiring of the underlying neuronal circuitry. Measurements of synaptic innervation, connection probabilities and subcellular organization of synaptic inputs are thus among the most active fields of research in contemporary neuroscience. Methods to measure these quantities range from electrophysiological recordings over reconstructions of dendrite-axon overlap at light-microscopic levels to dense circuit reconstructions of small volumes at electron-microscopic resolution. However, quantitative and complete measurements at subcellular resolution and mesoscopic scales to obtain all local and long-range synaptic in/outputs for any neuron within an entire brain region are beyond present methodological limits. Here, we present a novel concept, implemented within an interactive software environment called NeuroNet, which allows (i) integration of sparsely sampled (sub)cellular morphological data into an accurate anatomical reference frame of the brain region(s) of interest, (ii) up-scaling to generate an average dense model of the neuronal circuitry within the respective brain region(s) and (iii) statistical measurements of synaptic innervation between all neurons within the model. We illustrate our approach by generating a dense average model of the entire rat vibrissal cortex, providing the required anatomical data, and illustrate how to measure synaptic innervation statistically. Comparing our results with data from paired recordings in vitro and in vivo, as well as with reconstructions of synaptic contact sites at light- and electron-microscopic levels, we find that our in silico measurements are in line with previous results.

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 Dates: 2014-11
 Publication Status: Published online
 Pages: -
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 Rev. Method: Peer
 Identifiers: DOI: 10.3389/fnana.2014.00129
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Title: Frontiers in Neuroanatomy
  Abbreviation : Front Neuroanat
Source Genre: Journal
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Publ. Info: Lausanne, Switzerland : Frontiers Research Foundation
Pages: - Volume / Issue: 8 Sequence Number: 129 Start / End Page: 1 - 18 Identifier: ISSN: 1662-5129