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  The nuts and bolts of the Haloferax CRISPR-Cas system I-B.

Maier, L. K., Stachler, A. E., Brendel, J., Stoll, B., Fischer, S., Haas, K., et al. (2019). The nuts and bolts of the Haloferax CRISPR-Cas system I-B. RNA Biology, 16(4), 469-480. doi:10.1080/15476286.2018.1460994.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-26FB-A Version Permalink: http://hdl.handle.net/21.11116/0000-0003-9981-E
Genre: Journal Article

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 Creators:
Maier, L. K., Author
Stachler, A. E., Author
Brendel, J., Author
Stoll, B., Author
Fischer, S., Author
Haas, K., Author
Schwarz, T., Author
Alkhnbashi, O. S., Author
Sharma, K.1, Author              
Urlaub, H.1, Author              
Backofen, R., Author
Gophna, U., Author
Marchfelder, A., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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Free keywords: Archaea; CRISPR-Cas; CRISPRi; Haloarchaea; self-targeting; type I-B
 Abstract: Invading genetic elements pose a constant threat to prokaryotic survival, requiring an effective defence. Eleven years ago, the arsenal of known defence mechanisms was expanded by the discovery of the CRISPR-Cas system. Although CRISPR-Cas is present in the majority of archaea, research often focuses on bacterial models. Here, we provide a perspective based on insights gained studying CRISPR-Cas system I-B of the archaeon Haloferax volcanii. The system relies on more than 50 different crRNAs, whose stability and maintenance critically depend on the proteins Cas5 and Cas7, which bind the crRNA and form the Cascade complex. The interference activity requires a seed sequence and can interact with multiple PAM sequences. H. volcanii stands out as the first example of an organism that can tolerate autoimmunity via the CRISPR-Cas system while maintaining a constitutively active system. In addition, the H. volcanii system was successfully developed into a tool for gene regulation.

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Language(s): eng - English
 Dates: 2018-05-212019
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1080/15476286.2018.1460994
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Title: RNA Biology
Source Genre: Journal
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Pages: - Volume / Issue: 16 (4) Sequence Number: - Start / End Page: 469 - 480 Identifier: -