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  Molecular counting of membrane receptor subunits with single-molecule localization microscopy

Krüger, C., Fricke, F., Karathanasis, C., Dietz, M. S., Malkusch, S., Hummer, G., et al. (2017). Molecular counting of membrane receptor subunits with single-molecule localization microscopy. In Proceedings SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X. doi:10.1117/12.2256638.

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 Creators:
Krüger, Carmen1, Author
Fricke, Franziska1, Author
Karathanasis, Christos1, Author
Dietz, Marina S.1, Author
Malkusch, Sebastian1, Author
Hummer, Gerhard2, 3, Author                 
Heilemann, Mike1, Author
Affiliations:
1Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt am Main, Germany, ou_persistent22              
2Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society, ou_2068292              
3Institute of Biophysics, Goethe-University Frankfurt, 60438 Frankfurt am Main, Germany, ou_persistent22              

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Free keywords: single-molecule localization microscopy, super-resolution microscopy, photoswitchable fluorophores, photokinetic analysis, kinetic models for fluorophores, membrane protein stoichiometry
 Abstract: We report on quantitative single-molecule localization microscopy, a method that next to super-resolved images of cellular structures provides information on protein copy numbers in protein clusters. This approach is based on the analysis of blinking cycles of single fluorophores, and on a model-free description of the distribution of the number of blinking events. We describe the experimental and analytical procedures, present cellular data of plasma membrane proteins and discuss the applicability of this method

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Language(s): eng - English
 Dates: 2017-02-21
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1117/12.2256638
 Degree: -

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Title: SPIE BiOS, 2017
Place of Event: San Francisco, California, United States
Start-/End Date: 2017-01-28 - 2017-02-02
Invited: Yes

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Title: Proceedings SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X
Source Genre: Proceedings
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Publ. Info: -
Pages: - Volume / Issue: 10071 Sequence Number: 100710K Start / End Page: - Identifier: -