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  Strontium-modification of porous scaffolds from mineralized collagen for potential use in bone defect therapy

Quade, M., Schumacher, M., Bernhardt, A., Lode, A., Kampschulte, M., Voss, A., et al. (2018). Strontium-modification of porous scaffolds from mineralized collagen for potential use in bone defect therapy. Materials Science & Engineering C, 84, 159-167. doi:10.1016/j.msec.2017.11.038.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-2E99-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-3B01-C
Genre: Journal Article

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 Creators:
Quade, Mandy1, Author
Schumacher, Matthias1, Author
Bernhardt, Anne1, Author
Lode, Anja1, Author
Kampschulte, Marian1, Author
Voss, Andrea1, Author
Simon, Paul2, Author              
Uckermann, Ortrud1, Author
Kirsch, Matthias1, Author
Gelinsky, Michael1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Paul Simon, Chemical Metal Science, Max Planck Institute for Chemical Physics of Solids, Max Planck Society, ou_1863418              

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 Abstract: The present study describes the development and characterization of strontium(II)-modified biomimetic scaffolds based on mineralized collagen type I as potential biomaterial for the local treatment of defects in systemically impaired (e.g. osteoporotic) bone. In contrast to already described collagen/hydroxyapatite nanocomposites calcium was substituted with strontium to the extent of 25, 50, 75 and 100 mol% by substituting the CaCl2-stock solution (0.1 M) with SrCl2 (0.1 M) during the scaffold synthesis. Simultaneous fibrillation and mineralization of collagen led to the formation of collagen-mineral nanocomposites with mineral phases shifting from nanocrystalline hydroxyapatite (Sr0) over poorly crystalline Sr-rich phases towards a mixed mineral phase (Sr100), consisting of an amorphous strontium phosphate (identified as Collin's salt, Sr6H3(PO4)5 ∗ 2 H2O, CS) and highly crystalline strontium hydroxyapatite (Sr5(PO4)3OH, SrHA). The formed mineral phases were characterized by transmission electron microscopy (TEM) and RAMAN spectroscopy. All collagen/mineral nanocomposites with graded strontium content were processed to scaffolds exhibiting an interconnected porosity suitable for homogenous cell seeding in vitro. Strontium ions (Sr2 +) were released in a sustained manner from the modified scaffolds, with a clear correlation between the released Sr2 + concentration and the degree of Sr-substitution. The accumulated specific Sr2 + release over the course of 28 days reached 141.2 μg (~ 27 μg mg− 1) from Sr50 and 266.1 μg (~ 35 μg mg− 1) from Sr100, respectively. Under cell culture conditions this led to maximum Sr2 + concentrations of 0.41 mM (Sr50) and 0.73 mM (Sr100) measured on day 1, which declined to 0.08 mM and 0.16 mM, respectively, at day 28. Since Sr2 + concentrations in this range are known to have an osteo-anabolic effect, these scaffolds are promising biomaterials for the clinical treatment of defects in systemically impaired bone.

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Language(s): eng - English
 Dates: 2018-03-012018-03-01
 Publication Status: Published in print
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Title: Materials Science & Engineering C
Source Genre: Journal
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Publ. Info: Lausanne, Switzerland : Elsevier
Pages: - Volume / Issue: 84 Sequence Number: - Start / End Page: 159 - 167 Identifier: ISSN: 0928-4931
CoNE: /journals/resource/954926245533_1