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  A subset of chemosensory genes differs between two populations of a specialized leaf beetle after host plant shift

Wang, D., Pentzold, S., Kunert, M., Groth, M., Brandt, W., Pasteels, M., et al. (2018). A subset of chemosensory genes differs between two populations of a specialized leaf beetle after host plant shift. Ecology and Evolution, 8(16), 8055-8075. doi:10.1002/ece3.4246.

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http://dx.doi.org/10.1002/ece3.4246 (Publisher version)
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 Creators:
Wang, Ding1, 2, Author              
Pentzold, Stefan1, Author              
Kunert, Maritta1, Author              
Groth, M., Author
Brandt, W., Author
Pasteels, M., Author
Boland, Wilhelm1, Author              
Burse, Antje1, Author              
Affiliations:
1Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, ou_24028              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

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 Abstract: Due to its fundamental role in shaping host selection behavior, we have analyzed the chemosensory repertoire of Chrysomela lapponica. This specialized leaf beetle evolved distinct populations which shifted from the ancestral host plant, willow (Salix sp., Salicaceae), to birch (Betula rotundifolia, Betulaceae). We identified 114 chemosensory candidate genes in adult C. lapponica: 41 olfactory receptors (ORs), eight gustatory receptors, 17 ionotropic receptors, four sensory neuron membrane proteins, 32 odorant binding proteins (OBPs), and 12 chemosensory proteins (CSP) by RNA-seq. Differential expression analyses in the antennae revealed significant upregulation of one minus-C OBP (ClapOBP27) and one CSP (ClapCSP12) in the willow feeders. In contrast, one OR (ClapOR17), four minus-C OBPs (ClapOBP02, 07, 13, 20), and one plus-C OBP (ClapOBP32) were significantly upregulated in birch feeders. The differential expression pattern in the legs was more complex. To narrow down putative ligands acting as cues for host discrimination, the relative abundance and diversity of volatiles of the two host plant species were analyzed. In addition to salicylaldehyde (willow-specific), both plant species differed mainly in their emission rate of terpenoids such as (E,E)-α- farnesene (high in willow) or 4,8-dimethylnona- 1,3,7- triene (high in birch). Qualitatively, the volatiles were similar between willow and birch leaves constituting an “olfactory bridge” for the beetles. Subsequent structural modeling of the three most differentially expressed OBPs and docking studies using 22 host volatiles indicated that ligands bind with varying affinity. We suggest that the evolution of particularly minus-C OBPs and ORs in C. lapponica facilitated its host plant shift via chemosensation of the phytochemicals from birch as novel host plant.

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 Dates: 20172018-07-202018
 Publication Status: Published in print
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 Identifiers: Other: BOL696
DOI: 10.1002/ece3.4246
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Title: Ecology and Evolution
Source Genre: Journal
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Publ. Info: John Wiley & Sons, Inc.
Pages: - Volume / Issue: 8 (16) Sequence Number: - Start / End Page: 8055 - 8075 Identifier: ISSN: 2045-7758
CoNE: https://pure.mpg.de/cone/journals/resource/2045-7758