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  High frequency neural spiking and auditory signaling by ultrafast red-shifted optogenetics.

Mager, T., de la Morena, D. L., Senn, V., Schlotte, J., D´Errico, A., Feldbauer, K., et al. (2018). High frequency neural spiking and auditory signaling by ultrafast red-shifted optogenetics. Nature Communications, 9: 1750. doi:10.1038/s41467-018-04146-3.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-3E5E-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-912E-6
Genre: Journal Article

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 Creators:
Mager, T., Author
de la Morena, D. L., Author
Senn, V., Author
Schlotte, J., Author
D´Errico, A., Author
Feldbauer, K., Author
Wrobel, C., Author
Jung, S., Author
Bodensiek, K., Author
Rankovic, V., Author
Browne, L., Author
Huet, A., Author
Jüttner, J., Author
Wood, P. G., Author
Letzkus, J. J., Author
Moser, T.1, Author              
Bamberg, E., Author
Affiliations:
1Research Group of Synaptic Nanophysiology, MPI for Biophysical Chemistry, Max Planck Society, ou_2205655              

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 Abstract: Optogenetics revolutionizes basic research in neuroscience and cell biology and bears potential for medical applications. We develop mutants leading to a unifying concept for the construction of various channelrhodopsins with fast closing kinetics. Due to different absorption maxima these channelrhodopsins allow fast neural photoactivation over the whole range of the visible spectrum. We focus our functional analysis on the fast-switching, red light-activated Chrimson variants, because red light has lower light scattering and marginal phototoxicity in tissues. We show paradigmatically for neurons of the cerebral cortex and the auditory nerve that the fast Chrimson mutants enable neural stimulation with firing frequencies of several hundred Hz. They drive spiking at high rates and temporal fidelity with low thresholds for stimulus intensity and duration. Optical cochlear implants restore auditory nerve activity in deaf mice. This demonstrates that the mutants facilitate neuroscience research and future medical applications such as hearing restoration.

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Language(s): eng - English
 Dates: 2018-05-01
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-018-04146-3
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Title: Nature Communications
Source Genre: Journal
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Pages: 14 Volume / Issue: 9 Sequence Number: 1750 Start / End Page: - Identifier: -