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  A Peltier-cooled microscope stage for protein crystal post-crystallization treatment

Dietl, A., Kieser, C., & Barends, T. (2017). A Peltier-cooled microscope stage for protein crystal post-crystallization treatment. Journal of Applied Crystallography, 50(4), 1208-1211. doi:10.1107/S1600576717008755.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0001-4249-3 Version Permalink: https://hdl.handle.net/21.11116/0000-0001-610C-5
Genre: Journal Article

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JApplCryst_50_2017_1208.pdf (Any fulltext), 718KB
 
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https://onlinelibrary.wiley.com/doi/pdf/10.1107/S1600576717008755 (Any fulltext)
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 Creators:
Dietl, Andreas1, Author           
Kieser, Christian2, Author           
Barends, Thomas1, Author           
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
2Electronics Laboratory, Max Planck Institute for Medical Research, Max Planck Society, ou_persistent22              

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Free keywords: cryocrystallography; crystal quality; equipment; Peltier cooling
 Abstract: Crystals of the multi-enzyme complex hydrazine synthase showed severe diffuse scattering and high mosaicity. Improved diffraction quality was achieved by soaking the crystals in highly concentrated betaine solutions at reduced temperatures. To enable this, a Peltier-cooled microscope stage was developed for the slow cooling of protein crystals immersed in cryoprotectants or other soaking solutions. Both the construction of the stage and its successful application to hydrazine synthase crystals are described.

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Language(s): eng - English
 Dates: 2017-02-072017-06-122017-08-01
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1107/S1600576717008755
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Title: Journal of Applied Crystallography
  Abbreviation : J. Appl. Cryst.
Source Genre: Journal
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Publ. Info: Oxford, England : Blackwell Publishing on behalf of the International Union of Crystallography
Pages: - Volume / Issue: 50 (4) Sequence Number: - Start / End Page: 1208 - 1211 Identifier: ISSN: 0021-8898
CoNE: https://pure.mpg.de/cone/journals/resource/954925410812