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  The effect of auxin (indole-3-acetic acid) on the growth rate and tropism of the sporangiophore of Phycomyces blakesleeanus and identification of auxin-related genes

Živanović, B. D., Ullrich, K. K., Steffens, B., Spasić, S. Z., & Galland, P. (2018). The effect of auxin (indole-3-acetic acid) on the growth rate and tropism of the sporangiophore of Phycomyces blakesleeanus and identification of auxin-related genes. Protoplasma, 255(5), 1331-1347. doi:10.1007/s00709-018-1232-2.

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Genre: Journal Article

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 Creators:
Živanović, Branka D., Author
Ullrich, Kristian K.1, Author              
Steffens, Bianka, Author
Spasić, Sladjana Z., Author
Galland, Paul, Author
Affiliations:
1Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              

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Free keywords: Auxin; Auxin-related genes; Growth rate; Phycomyces; Sporangiophore; Tropism
 Abstract: The roles of fungal auxins in the regulation of elongation growth, photo-, and gravitropism are completely unknown. We analyzed the effects of exogenous IAA (indole-3-acetic acid), various synthetic auxins including 1-NAA (1-naphthaleneacetic acid) and 2,4-D (2,4-dichlorophenoxyacetic acid), and the auxin transport inhibitor NPA (N-1-naphtylphtalamic acid) on the growth rate and bending of the unicellular sporangiophore of the zygomycete fungus, Phycomyces blakesleeanus. Sporangiophores that were submerged in an aqueous buffer responded to IAA with a sustained enhancement of the growth rate, while 1-NAA, 2,4-D, and NPA elicited an inhibition. In contrast, sporangiophores kept in air responded to IAA with a 20 to 40% decrease of the growth rate, while 1-NAA and NPA elicited an enhancement. The unilateral and local application of IAA in the growing zone of the sporangiophore elicited in 30 min a moderate negative tropic bending in wild type C2 and mutant C148madC, which was, however, partially masked by a concomitant avoidance response caused by the aqueous buffer. Auxin transport-related genes ubiquitous in plants were found in a BLAST search of the Phycomyces genome. They included members of the AUX1 (auxin influx carrier protein 1), PILS (PIN-LIKES, auxin transport facilitator protein), and ABCB (plant ATP-binding cassette transporter B) families while members of the PIN family were absent. Our observations imply that IAA represents an intrinsic element of the sensory transduction of Phycomyces and that its mode of action must very likely differ in several respects from that operating in plants.

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Language(s): eng - English
 Dates: 2017-09-182018-02-262018-03-092018-09
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/s00709-018-1232-2
 Degree: -

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Project name : Serbian Ministry of Education, Science and Technological Development
Grant ID : 173040
Funding program : Deutsche Forschungsgemeinschaft (GA 173/13-1)
Funding organization : -
Project name : -
Grant ID : -
Funding program : DLR/BMWi (50WB1325)
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Title: Protoplasma
Source Genre: Journal
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Publ. Info: Wien : Springer-Verlag
Pages: - Volume / Issue: 255 (5) Sequence Number: - Start / End Page: 1331 - 1347 Identifier: ISSN: 0033-183X
CoNE: https://pure.mpg.de/cone/journals/resource/954925435463