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  Xpo7 is a broad-spectrum exportin and a nuclear import receptor.

Aksu, M., Pleiner, T., Karaca, S., Kappert, C., Dehne, H. J., Seibel, K., et al. (2018). Xpo7 is a broad-spectrum exportin and a nuclear import receptor. Journal of Cell Biology, 217(7), 2329-2340. doi:10.1083/jcb.201712013.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-46CC-B Version Permalink: http://hdl.handle.net/21.11116/0000-0003-1AA7-4
Genre: Journal Article

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 Creators:
Aksu, M.1, Author              
Pleiner, T.1, Author              
Karaca, S.2, Author              
Kappert, C.1, Author              
Dehne, H. J.1, Author              
Seibel, K.1, Author              
Urlaub, H.2, Author              
Bohnsack, M. T., Author
Görlich, D.1, Author              
Affiliations:
1Department of Cellular Logistics, MPI for Biophysical Chemistry, Max Planck Society, ou_578574              
2Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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 Abstract: Exportins bind cargo molecules in a RanGTP-dependent manner inside nuclei and transport them through nuclear pores to the cytoplasm. CRM1/Xpo1 is the best-characterized exportin because specific inhibitors such as leptomycin B allow straightforward cargo validations in vivo. The analysis of other exportins lagged far behind, foremost because no such inhibitors had been available for them. In this study, we explored the cargo spectrum of exportin 7/Xpo7 in depth and identified not only ∼200 potential export cargoes but also, surprisingly, ∼30 nuclear import substrates. Moreover, we developed anti-Xpo7 nanobodies that acutely block Xpo7 function when transfected into cultured cells. The inhibition is pathway specific, mislocalizes export cargoes of Xpo7 to the nucleus and import substrates to the cytoplasm, and allowed validation of numerous tested cargo candidates. This establishes Xpo7 as a broad-spectrum bidirectional transporter and paves the way for a much deeper analysis of exportin and importin function in the future.

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Language(s): eng - English
 Dates: 2018-05-102018-07-02
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1083/jcb.201712013
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Title: Journal of Cell Biology
Source Genre: Journal
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Pages: - Volume / Issue: 217 (7) Sequence Number: - Start / End Page: 2329 - 2340 Identifier: -