English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Photoactivatable rhodamine spiroamides and diazoketones decorated with “universal hydrophilizer” or hydroxyl groups.

Roubinet, B., Bischoff, M., Nizamov, S., Yan, S., Geisler, C., Stoldt, S., et al. (2018). Photoactivatable rhodamine spiroamides and diazoketones decorated with “universal hydrophilizer” or hydroxyl groups. The Journal of Organic Chemistry, 83(12), 6466-6476. doi:10.1021/acs.joc.8b00756.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0001-479F-D Version Permalink: http://hdl.handle.net/21.11116/0000-0003-B13E-0
Genre: Journal Article

Files

show Files
hide Files
:
2586363.pdf (Publisher version), 3MB
 
File Permalink:
-
Name:
2586363.pdf
Description:
-
Visibility:
Restricted ( Max Planck Society (every institute); )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2586363_Suppl.pdf (Supplementary material), 8MB
Name:
2586363_Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Roubinet, B.1, Author              
Bischoff, M., Author
Nizamov, S.1, Author              
Yan, S., Author
Geisler, C., Author
Stoldt, S.2, Author              
Mitronova, G.1, Author              
Belov, V. N.1, Author              
Bossi, M. L.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              
2Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              

Content

show
hide
Free keywords: -
 Abstract: Photoactivatable rhodamine spiroamides and spirocyclic diazoketones emerged recently as synthetic markers applicable in multicolor superresolution microscopy. However, their applicability in single molecule localization microscopy (SMLM) is often limited by aggregation, unspecific adhesion and low reactivity caused by insufficient solubility and precipitation from aqueous solutions. We report here two synthetic modifications increasing the polarity of compact polycyclic and hydrophobic labels decorated with a reactive group: attachment of 3-sulfo-L-alanyl - beta-alanine dipeptide (a "universal hydrophilizer") or allylic hydroxylation in photosensitive rhodamine diazoketones (and spiroamides). The superresolution images of tubulin and keratin filaments in fixed and living cells exemplify the performance of "blinking" spiroamides derived from N,N,N',N'-tetramethyl rhodamine.

Details

show
hide
Language(s): eng - English
 Dates: 2018-05-112018-06-15
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1021/acs.joc.8b00756
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Journal of Organic Chemistry
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 83 (12) Sequence Number: - Start / End Page: 6466 - 6476 Identifier: -