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  Quantifying absolute addressability in DNA origami with molecular resolution

Strauss, M. T., Schueder, F., Haas, D., Nickels, P. C., & Jungmann, R. (2018). Quantifying absolute addressability in DNA origami with molecular resolution. Nature Communications, 9: 1600. doi:10.1038/s41467-018-04031-z.

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 Creators:
Strauss, Maximilian T.1, Author              
Schueder, Florian1, Author              
Haas, Daniel1, Author              
Nickels, Philipp C.1, Author              
Jungmann, Ralf1, Author              
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              

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Free keywords: SUPERRESOLUTION MICROSCOPY; NANOSCALE SHAPES; FOLDING DNA; NANOSTRUCTURES; RECONSTRUCTION; FLUORESCENCE; SIMULATION; DESIGN; MOTORScience & Technology - Other Topics;
 Abstract: Self-assembled DNA nanostructures feature an unprecedented addressability with sub-nanometer precision and accuracy. This addressability relies on the ability to attach functional entities to single DNA strands in these structures. The efficiency of this attachment depends on two factors: incorporation of the strand of interest and accessibility of this strand for downstream modification. Here we use DNA-PAINT super-resolution microscopy to quantify both incorporation and accessibility of all individual strands in DNA origami with molecular resolution. We find that strand incorporation strongly correlates with the position in the structure, ranging from a minimum of 48% on the edges to a maximum of 95% in the center. Our method offers a direct feedback for the rational refinement of the design and assembly process of DNA nanostructures and provides a long sought-after quantitative explanation for efficiencies of DNA-based nanomachines.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published online
 Pages: 7
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 Table of Contents: -
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 9 Sequence Number: 1600 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723