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  Structural rearrangements of the histone octamer translocate DNA

Bilokapic, S., Strauss, M., & Halic, M. (2018). Structural rearrangements of the histone octamer translocate DNA. Nature Communications, 9: 1330. doi:10.1038/s41467-018-03677-z.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-DD43-B Version Permalink: http://hdl.handle.net/21.11116/0000-0001-DD44-A
Genre: Journal Article

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 Creators:
Bilokapic, Silvija1, Author
Strauss, Mike2, Author              
Halic, Mario1, Author
Affiliations:
1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: NUCLEOSOME CORE PARTICLE; RNA-POLYMERASE-II; CRYSTAL-STRUCTURE; SIN MUTATIONS; CRYO-EM; H4 TAIL; CHROMATIN; ISWI; ACCESSIBILITY; MECHANISMSScience & Technology - Other Topics;
 Abstract: Nucleosomes, the basic unit of chromatin, package and regulate expression of eukaryotic genomes. Nucleosomes are highly dynamic and are remodeled with the help of ATP-dependent remodeling factors. Yet, the mechanism of DNA translocation around the histone octamer is poorly understood. In this study, we present several nucleosome structures showing histone proteins and DNA in different organizational states. We observe that the histone octamer undergoes conformational changes that distort the overall nucleosome structure. As such, rearrangements in the histone core a-helices and DNA induce strain that distorts and moves DNA at SHL 2. Distortion of the nucleosome structure detaches histone a-helices from the DNA, leading to their rearrangement and DNA translocation. Biochemical assays show that cross-linked histone octamers are immobilized on DNA, indicating that structural changes in the octamer move DNA. This intrinsic plasticity of the nucleosome is exploited by chromatin remodelers and might be used by other chromatin machineries.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 9 Sequence Number: 1330 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723