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Language(s):
eng - English
Dates:
2107-052017-05
Publication Status:
Issued
Pages:
73
Publishing info:
Kiel : Christian-Albrechts-Universität zu Kiel
Table of Contents:
Table of Contents
1. Summary ....................................................................................................................................... 1
2. Introduction ................................................................................................................................... 2
2.1. Origin of Z. tritici .................................................................................................................. 3
2.2. Genome organization of Z. tritici .......................................................................................... 3
2.3. Z. tritici infection Cycle ........................................................................................................ 3
2.4. Role of Promoters in Regulation of Gene Expression .......................................................... 5
2.5. Properties of Eukaryotic Promoters ...................................................................................... 6
2.6. Why promoter evolution should be studied .......................................................................... 8
2.7. Population genomics Study ................................................................................................... 8
2.8. Nucleotide Diversity and neutrality test ................................................................................ 9
2.9. Aim of the study .................................................................................................................. 11
3. Material and Methods ................................................................................................................. 12
3.1. Assembly of Z. tritici isolates.............................................................................................. 12
3.2. Multiple genome alignments ............................................................................................... 12
3.3. Processing multiple genome alignment using MaFilter ...................................................... 12
3.4. Predicting fungal effector proteins using EffectorP ............................................................ 13
3.5. Statistical analysis ............................................................................................................... 13
3.6. Correlation study ................................................................................................................. 14
4. Results......................................................................................................................................... 16
4.1. De-novo assembly of short Illumina reads .......................................................................... 16
4.2. Filtering of multiple genome alignment .............................................................................. 16
4.3. Genetic diversity and test of neutrality................................................................................ 17
4.4. Negative correlation between nucleotide diversity of promoters and level of gene expression....................................................................................................................................... 22
5. Discussion ................................................................................................................................... 25
6. Conclusion and future prospects ................................................................................................. 28
7. Bibliography ............................................................................................................................... 29
8. Acknowledgement ...................................................................................................................... 35
9. Supplementary Data .................................................................................................................... 36
Rev. Type:
-
Identifiers:
Other: Dipl/12917
Degree:
Master
