English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High-speed 4D computational microscopy of bacterial surface motility

De Anda, J., Lee, E. Y., Lee, C. K., Bennett, R. R., Ji, X., Soltani, S., et al. (2017). High-speed 4D computational microscopy of bacterial surface motility. ACS Nano, 11(9), 9340-9351. doi:10.1021/acsnano.7b04738.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0001-ADE2-D Version Permalink: http://hdl.handle.net/21.11116/0000-0001-ADE3-C
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
De Anda, J., Author
Lee, E. Y., Author
Lee, C. K., Author
Bennett, R. R., Author
Ji, X., Author
Soltani, S., Author
Harrison, M. C., Author
Baker, A. E., Author
Luo, Y., Author
Chou, T., Author
O'Toole, G. A., Author
Armani, A. M., Author
Golestanian, R.1, Author              
Wong, G. C. L., Author
Affiliations:
1Department of Living Matter Physics, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2570692              

Content

show
hide
Free keywords: -
 Abstract: Bacteria exhibit surface motility modes that play pivotal roles in early-stage biofilm community development, such as type IV pili-driven "twitching" motility and flagellum-driven "spinning" and "swarming" motility. Appendage-driven motility is controlled by molecular motors, and analysis of surface motility behavior is complicated by its inherently 3D nature, the speed of which is too fast for confocal microscopy to capture. Here, we combine electromagnetic field computation and statistical image analysis to generate 3D movies close to a surface at 5 ms time resolution using conventional inverted microscopes. We treat each bacterial cell as a spherocylindrical lens and use finite element modeling to solve Maxwell's equations and compute the diffracted light intensities associated with different angular orientations of the bacterium relative to the surface. By performing cross-correlation calculations between measured 2D microscopy images and a library of computed light intensities, we demonstrate that near-surface 3D movies of Pseudomonas aeruginosa translational and rotational motion are possible at high temporal resolution. Comparison between computational reconstructions and detailed hydrodynamic calculations reveals that P. aeruginosa act like low Reynolds number spinning tops with unstable orbits, driven by a flagellum motor with a torque output of ~2 pN μm. Interestingly, our analysis reveals that P. aeruginosa can undergo complex flagellum-driven dynamical behavior, including precession, nutation, and an unexpected taxonomy of surface motility mechanisms, including upright-spinning bacteria that diffuse laterally across the surface, and horizontal bacteria that follow helicoidal trajectories and exhibit superdiffusive movements parallel to the surface. © 2017 American Chemical Society.

Details

show
hide
Language(s): eng - English
 Dates: 2017-08-242017
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1021/acsnano.7b04738
BibTex Citekey: DeAnda2017
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ACS Nano
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 11 (9) Sequence Number: - Start / End Page: 9340 - 9351 Identifier: -