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  Exothermicity is not a necessary condition for enhanced diffusion of enzymes

Illien, P., Zhao, X., Dey, K. K., Butler, P. J., Sen, A., & Golestanian, R. (2017). Exothermicity is not a necessary condition for enhanced diffusion of enzymes. Nano Letters, 17(7), 4415-4420. doi:10.1021/acs.nanolett.7b01502.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-73E5-B Version Permalink: http://hdl.handle.net/21.11116/0000-0001-73E6-A
Genre: Journal Article

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 Creators:
Illien, P., Author
Zhao, X., Author
Dey, K. K., Author
Butler, P. J., Author
Sen, A., Author
Golestanian, Ramin1, Author              
Affiliations:
1Department of Living Matter Physics, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2570692              

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 Abstract: Recent experiments have revealed that the diffusivity of exothermic and fast enzymes is enhanced when they are catalytically active, and different physical mechanisms have been explored and quantified to account for this observation. We perform measurements on the endothermic and relatively slow enzyme aldolase, which also shows substrate-induced enhanced diffusion. We propose a new physical paradigm, which reveals that the diffusion coefficient of a model enzyme hydrodynamically coupled to its environment increases significantly when undergoing changes in conformational fluctuations in a substrate concentration dependent manner, and is independent of the overall turnover rate of the underlying enzymatic reaction. Our results show that substrate-induced enhanced diffusion of enzyme molecules can be explained within an equilibrium picture and that the exothermicity of the catalyzed reaction is not a necessary condition for the observation of this phenomenon. © 2017 American Chemical Society.

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Language(s): eng - English
 Dates: 2017-06-082017
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1021/acs.nanolett.7b01502
BibTex Citekey: Illien2017b
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Title: Nano Letters
Source Genre: Journal
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Pages: - Volume / Issue: 17 (7) Sequence Number: - Start / End Page: 4415 - 4420 Identifier: -