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  L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast

Hochrein, L., Mitchell, L. A., Schulz, K., Messerschmidt, K., & Mueller-Roeber, B. (2018). L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast. Nature Communications, 9(1): 1931. doi:10.1038/s41467-017-02208-6.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-5D8F-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-5D90-4
Genre: Journal Article

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 Creators:
Hochrein, Lena1, Author
Mitchell, Leslie A.1, Author
Schulz, Karina1, Author
Messerschmidt, Katrin1, Author
Mueller-Roeber, B.2, Author              
Affiliations:
1external, ou_persistent22              
2Transcription Factors and Gene Regulatory Networks, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753316              

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 Abstract: The synthetic yeast genome constructed by the International Synthetic Yeast Sc2.0 consortium adds thousands of loxPsym recombination sites to all 16 redesigned chromosomes, allowing the shuffling of Sc2.0 chromosome parts by the Cre-loxP recombination system thereby enabling genome evolution experiments. Here, we present L-SCRaMbLE, a light-controlled Cre recombinase for use in the yeast Saccharomyces cerevisiae. L-SCRaMbLE allows tight regulation of recombinase activity with up to 179-fold induction upon exposure to red light. The extent of recombination depends on induction time and concentration of the chromophore phycocyanobilin (PCB), which can be easily adjusted. The tool presented here provides improved recombination control over the previously reported estradiol-dependent SCRaMbLE induction system, mediating a larger variety of possible recombination events in SCRaMbLE-ing a reporter plasmid. Thereby, L-SCRaMbLE boosts the potential for further customization and provides a facile application for use in the S. cerevisiae genome re-engineering project Sc2.0 or in other recombination-based systems.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: URI: https://doi.org/10.1038/s41467-017-02208-6
Other: Hochrein2018
DOI: 10.1038/s41467-017-02208-6
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: 1931 Volume / Issue: 9 (1) Sequence Number: 1931 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723