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  Systematic transcriptomics reveals a biphasic mode of sarcomere morphogenesis in flight muscles regulated by Spalt

Spletter, M. L., Barz, C., Yeroslaviz, A., Zhang, X., Lemke, S. B., Brunner, E., et al. (2017). Systematic transcriptomics reveals a biphasic mode of sarcomere morphogenesis in flight muscles regulated by Spalt. bioRxiv, 229534. doi:10.1101/229534.

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Spletter, Maria L.1, Author              
Barz, Christiane1, Author              
Yeroslaviz, Assa2, Author              
Zhang, Xu3, Author
Lemke, Sandra B.1, Author              
Brunner, Erich3, Author
Cardone, Giovanni4, Author              
Basler, Konrad3, Author
Habermann, Bianca H.1, Author              
Schnorrer, Frank1, Author
Affiliations:
1Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565168              
2Habermann, Bianca / Computational Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1832284              
3External Organizations, ou_persistent22              
4Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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 Abstract: Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study how sarcomeres are built, we performed transcriptome sequencing of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, two clusters are strongly enriched for sarcomeric components. Temporal gene expression together with detailed morphological analysis enabled us to define two distinct phases of sarcomere development, which both require the transcriptional regulator Spalt major. During the sarcomere formation phase, 1.8 micrometer long immature sarcomeres assemble myofibrils that spontaneously contract. During the sarcomere maturation phase, these sarcomeres grow to their final 3.2 micrometer length and 1.5 micrometer diameter and acquire stretch-sensitivity. Interestingly, the final number of myofibrils per flight muscle fiber is determined at the onset of the first phase. Together, this defines a biphasic mode of sarcomere and myofibril morphogenesis - a new concept that may also apply to vertebrate muscle or heart development.

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 Dates: 2017-12-05
 Publication Status: Published online
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 Identifiers: DOI: 10.1101/229534
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Title: bioRxiv
Source Genre: Journal
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Pages: - Volume / Issue: - Sequence Number: 229534 Start / End Page: - Identifier: -