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  Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides

Sallin, O., Reymond, L., Gondrand, C., Raith, F., Koch, B., & Johnsson, K. (2018). Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. eLife, 7, 1-52. doi:10.7554/eLife.32638.

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 Urheber:
Sallin , Olivier, Autor
Reymond, Luc1, Autor           
Gondrand , Corentin, Autor
Raith , Fabio, Autor
Koch , Birgit, Autor
Johnsson, Kai2, Autor           
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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 Zusammenfassung: We introduce a new class of semisynthetic fluorescent biosensors for the quantification of free nicotinamide adenine dinucleotide (NAD+) and ratios of reduced to oxidized nicotinamide adenine dinucleotide phosphate (NADPH/NADP+) in live cells. Sensing is based on controlling the spatial proximity of two synthetic fluorophores by binding of NAD(P) to the protein component of the sensor. The sensors possess a large dynamic range, can be excited at long wavelengths, are pH-insensitive, have tunable response range and can be localized in different organelles. Ratios of free NADPH/NADP+ are found to be higher in mitochondria compared to those found in the nucleus and the cytosol. By recording free NADPH/NADP+ ratios in response to changes in environmental conditions, we observe how cells can react to such changes by adapting metabolic fluxes. Finally, we demonstrate how a comparison of the effect of drugs on cellular NAD(P) levels can be used to probe mechanisms of action.

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Sprache(n): eng - English
 Datum: 2018-05-29
 Publikationsstatus: Erschienen
 Seiten: 52
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 Art der Begutachtung: Expertenbegutachtung
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Titel: eLife
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Cambridge : eLife Sciences Publications
Seiten: - Band / Heft: 7 Artikelnummer: - Start- / Endseite: 1 - 52 Identifikator: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X