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  MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution.

Eilers, Y., Ta, H., Gwosch, K., Balzarotti, F., & Hell, S. W. (2018). MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution. Proceedings of the National Academy of Sciences of the United States of America, 115(24), 6117-6122. doi:10.1073/pnas.1801672115.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-6965-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-4EEF-A
Genre: Journal Article

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 Creators:
Eilers, Y.1, Author              
Ta, H.1, Author              
Gwosch, K.1, Author              
Balzarotti, F.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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Free keywords: MINFLUX; localization; single molecule; tracking
 Abstract: Compared with localization schemes solely based on evaluating patterns of molecular emission, the recently introduced single-molecule localization concept called MINFLUX and the fluorescence nanoscopies derived from it require up to orders of magnitude fewer emissions to attain single-digit nanometer resolution. Here, we demonstrate that the lower number of required fluorescence photons enables MINFLUX to detect molecular movements of a few nanometers at a temporal sampling of well below 1 millisecond. Using fluorophores attached to thermally fluctuating DNA strands as model systems, we demonstrate that measurement times as short as 400 microseconds suffice to localize fluorescent molecules with ∼2-nm precision. Such performance is out of reach for popular camera-based localization by centroid calculation of emission diffraction patterns. Since theoretical limits have not been reached, our results show that emerging MINFLUX nanoscopy bears great potential for dissecting the motions of individual (macro)molecules at hitherto-unattained combinations of spatial and temporal resolution.

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Language(s): eng - English
 Dates: 2018-05-29
 Publication Status: Published online
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1801672115
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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 115 (24) Sequence Number: - Start / End Page: 6117 - 6122 Identifier: -