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  Targeting repair pathways with small molecules increases precise genome editing in pluripotent stem cells

Riesenberg, S., & Maricic, T. (2018). Targeting repair pathways with small molecules increases precise genome editing in pluripotent stem cells. Nature Communications, 9: 2164. doi:10.1038/s41467-018-04609-7.

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Riesenberg_Targeted_NatComm_2018.pdf (Verlagsversion), 2MB
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2018
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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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 Urheber:
Riesenberg, Stephan1, 2, Autor                 
Maricic, Tomislav2, Autor                 
Affiliations:
1The Leipzig School of Human Origins (IMPRS), Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_1497688              
2Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_1497672              

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 Zusammenfassung: Small molecule inhibitors can influence the choice of repair pathways, enhancing nucleotide substitution and gene integration in CRISPR-mediated genome editing. Here the authors introduce CRISPY, a mix of small molecules that can enhance precise editing with Cpf1 and Cas9D10A in hiPSCs.

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Sprache(n): eng - English
 Datum: 2018-06-04
 Publikationsstatus: Online veröffentlicht
 Seiten: 9
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1038/s41467-018-04609-7
 Art des Abschluß: -

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Titel: Nature Communications
Genre der Quelle: Zeitschrift
 Urheber:
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Ort, Verlag, Ausgabe: London : Nature Publishing Group UK
Seiten: - Band / Heft: 9 Artikelnummer: 2164 Start- / Endseite: - Identifikator: ISSN: 2041-1723