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  Morphological and transcriptomic analyses reveal three discrete primary stages of postembryonic development in the common fire salamander, Salamandra salamandra

Sanchez, E., Küpfer, E., Goedbloed, D. J., Nolte, A. W., Lüddecke, T., Schulz, S., et al. (2018). Morphological and transcriptomic analyses reveal three discrete primary stages of postembryonic development in the common fire salamander, Salamandra salamandra. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 330(2), 96-108. doi:10.1002/jez.b.22792.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-6B2F-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-6B30-1
Genre: Journal Article

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Sanchez, Eugenia, Author
Küpfer, Eliane, Author
Goedbloed, Daniel J., Author
Nolte, Arne W.1, Author              
Lüddecke, Tim, Author
Schulz, Stefan, Author
Vences, Miguel, Author
Steinfartz, Sebastian, Author
Affiliations:
1Research Group Evolutionary Genetics of Fishes, Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445645              

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Free keywords: amphibian metamorphosis evolution; gene expression; pigments (melanin and pteridines) biosynthesis; salamander steroidal alkaloids; skin restructuring; tail fin resorption
 Abstract: The postembryonic development of amphibians has been characterized as divided into three predominant periods, hereafter named primary developmental stages: premetamorphosis (PreM), prometamorphosis (ProM), metamorphic climax (Meta), and completion of metamorphosis (PostM), largely based on examination of anuran development. Here, we categorized the postembryonic development of larvae of a poisonous fire salamander (Salamandra salamandra) by integrating morphology and gene expression (transcriptomic) data. Morphological analysis revealed three distinct clusters suggestive of PreM, ProM, and Meta, which were confirmed in parallel by microarray‐derived gene expression analysis. In total, 3,510 probes targeted transcripts differentially expressed between the clusters we identified. Genes upregulated in PreM related to organogenesis, and those upregulated in Meta underlie structural proteins and related to development of anatomical structures and pigmentation. Biosynthesis pathways of pigments (pteridines and melanin) were upregulated during late ProM and Meta. Gas chromatographic analysis of alkaloids indicated the onset of steroidal alkaloid biosynthesis at ProM. When comparing gene expression in the fire salamander to that in other amphibians—three anurans, Xenopus laevis, X. tropicalis, and Michrohyla fissipes, and one caudate, Ambystoma mexicanum— we identified genes with conserved expression patterns involved in basic metamorphic processes such as skin restructuring and tail fin resorption. Our results support that primary stages of postembryonic development in caudates are homologous to those of anurans, and offer a baseline for the study of the evolution of developmental modes.

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Language(s): eng - English
 Dates: 2017-12-142017-07-252018-01-252018-03-05
 Publication Status: Published online
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 Identifiers: DOI: 10.1002/jez.b.22792
BibTex Citekey: doi:10.1002/jez.b.22792
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Title: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Source Genre: Journal
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Pages: - Volume / Issue: 330 (2) Sequence Number: - Start / End Page: 96 - 108 Identifier: -