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  Positively selected effector genes and their contribution to virulence in the smut fungus Sporisorium reilianum

Schweizer, G., Münch, K., Mannhaupt, G., Schirawski, J., Kahmann, R., & Dutheil, J. Y. (2018). Positively selected effector genes and their contribution to virulence in the smut fungus Sporisorium reilianum. Genome Biology and Evolution, 10(2), 629-645. doi:10.1093/gbe/evy023.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-6BE0-A Version Permalink: http://hdl.handle.net/21.11116/0000-0002-B52D-0
Genre: Journal Article

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 Creators:
Schweizer, Gabriel, Author
Münch, Karin, Author
Mannhaupt, Gertrud, Author
Schirawski, Jan, Author
Kahmann, Regine, Author
Dutheil, Julien Y.1, Author              
Affiliations:
1Research Group Molecular Systems Evolution, Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2068287              

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Free keywords: positive selection; effector evolution; smut fungi; comparative genomics; virulence
 Abstract: Plants and fungi display a broad range of interactions in natural and agricultural ecosystems ranging from symbiosis to parasitism. These ecological interactions result in coevolution between genes belonging to different partners. A well-understood example is secreted fungal effector proteins and their host targets, which play an important role in pathogenic interactions. Biotrophic smut fungi (Basidiomycota) are well-suited to investigate the evolution of plant pathogens, because several reference genomes and genetic tools are available for these species. Here, we used the genomes of Sporisorium reilianum f. sp. zeae and S. reilianum f. sp. reilianum, two closely related formae speciales infecting maize and sorghum, respectively, together with the genomes of Ustilago hordei, Ustilago maydis, and Sporisorium scitamineum to identify and characterize genes displaying signatures of positive selection. We identified 154 gene families having undergone positive selection during species divergence in at least one lineage, among which 77 were identified in the two investigated formae speciales of S. reilianum. Remarkably, only 29 of positively selected genes encode predicted secreted proteins. We assessed the contribution to virulence of nine of these candidate effector genes in S. reilianum f. sp. zeae by deleting individual genes, including a homologue of the effector gene pit2 previously characterized in U. maydis. Only the pit2 deletion mutant was found to be strongly reduced in virulence. Additional experiments are required to understand the molecular mechanisms underlying the selection forces acting on the other candidate effector genes, as well as the large fraction of positively selected genes encoding predicted cytoplasmic proteins. © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

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Language(s): eng - English
 Dates: 2018-01-292018
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: DOI: 10.1093/gbe/evy023
BibTex Citekey: Schweizer2018629
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Title: Genome Biology and Evolution
  Other : GBE
  Abbreviation : Genome Biol Evol
Source Genre: Journal
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Publ. Info: Oxford : Oxford Univ. Press
Pages: - Volume / Issue: 10 (2) Sequence Number: - Start / End Page: 629 - 645 Identifier: Other: 1759-6653
CoNE: /journals/resource/1759-6653