English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
 Local TagsRelease HistoryDetailsSummary
  Systems analysis reveals high genetic and antigen-driven predetermination of antibody repertoires throughout B Cell development

Greiff, V., Menzel, U., Miho, E., Weber, C., Riedel, re, R., Cook, S., et al. (2017). Systems analysis reveals high genetic and antigen-driven predetermination of antibody repertoires throughout B Cell development. Cell Reports, 19(7), 1467-1478. doi:10.1016/j.celrep.2017.04.054.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-0001-7066-E Version Permalink: https://hdl.handle.net/21.11116/0000-0004-42AA-2
Genre: Journal Article

Files

show Files
hide Files
:
1-s2.0-S221112471730565X-main.pdf (Publisher version), 4MB
View   Save
File Permalink:
https://hdl.handle.net/21.11116/0000-0001-7068-C
Name:
1-s2.0-S221112471730565X-main.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
http://creativecommons.org/licenses/by-nc-nd/4.0/

Locators

show
hide
Locator:
Link (Publisher version)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Greiff, Victor1, Author
Menzel, Ulrike, Author
Miho, Enkelejda, Author
Weber, Cédric, Author
Riedel, re, René, Author
Cook, Skylar, Author
Valai, Atijeh, Author
Lopes, Telma, Author
Radbruch, Andreas, Author
Winkler, Thomas H., Author
Reddy, Sai T., Author
Affiliations:
1External Organizations, ou_persistent22              

Content

show
hide
Free keywords: systems immunology; Ig-seq; bioinformatics
 Abstract: Antibody repertoire diversity and plasticity is crucial for broad protective immunity. Repertoires change in size and diversity across multiple B cell developmental stages and in response to antigen exposure. However, we still lack fundamental quantitative understanding of the extent to which repertoire diversity is predetermined. Therefore, we implemented a systems immunology framework for quantifying repertoire predetermination on three distinct levels: (1) B cell development (pre-B cell, naive B cell, plasma cell), (2) antigen exposure (three structurally different proteins), and (3) four antibody repertoire components (V-gene usage, clonal expansion, clonal diversity, repertoire size) extracted from antibody repertoire sequencing data (400 million reads). Across all three levels, we detected a dynamic balance of high genetic (e.g., >90 for V-gene usage and clonal expansion in naive B cells) and antigen-driven (e.g., 40 for clonal diversity in plasma cells) predetermination and stochastic variation. Our study has implications for the prediction and manipulation of humoral immunity.

Details

show
hide
Language(s): eng - English
 Dates: 2017-03-212016-07-072017-04-192017-05-162017
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.celrep.2017.04.054
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cell Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Maryland Heights, MO : Cell Press
Pages: - Volume / Issue: 19 (7) Sequence Number: - Start / End Page: 1467 - 1478 Identifier: ISSN: 2211-1247
CoNE: https://pure.mpg.de/cone/journals/resource/2211-1247