English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  STED nanoscopy of the centrosome linker reveals a CEP68-organized, periodic rootletin network anchored to a C-Nap1 ring at centrioles.

Vlijm, R., Li, X., Panic, M., Rüthnick, D., Hata, S., Herrmannsdörfer, F., et al. (2018). STED nanoscopy of the centrosome linker reveals a CEP68-organized, periodic rootletin network anchored to a C-Nap1 ring at centrioles. Proceedings of the National Academy of Sciences of the United States of America, 115(10), E2246-E2253. doi:10.1073/pnas.1716840115.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0001-73AB-D Version Permalink: http://hdl.handle.net/21.11116/0000-0003-BE47-8
Genre: Journal Article

Files

show Files
hide Files
:
2599137.pdf (Publisher version), 3MB
Name:
2599137.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2599137_Suppl.DCSupplemental (Supplementary material), 85KB
Name:
2599137_Suppl.DCSupplemental
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/xhtml+xml / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Vlijm, R., Author
Li, X., Author
Panic, M., Author
Rüthnick, D., Author
Hata, S., Author
Herrmannsdörfer, F., Author
Kuner, T., Author
Heilemann, M., Author
Engelhardt, Johann, Author
Hell, S. W.1, Author              
Schiebel, E., Author
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

Content

show
hide
Free keywords: centrosome; centrosome linker; STED superresolution; rootletin; CEP68
 Abstract: The centrosome linker proteins C-Nap1, rootletin, and CEP68 connect the two centrosomes of a cell during interphase into one microtubule-organizing center. This coupling is important for cell migration, cilia formation, and timing of mitotic spindle formation. Very little is known about the structure of the centrosome linker. Here, we used stimulated emission depletion (STED) microscopy to show that each C-Nap1 ring at the proximal end of the two centrioles organizes a rootletin ring and, in addition, multiple rootletin/CEP68 fibers. Rootletin/CEP68 fibers originating from the two centrosomes form a web-like, interdigitating network, explaining the flexible nature of the centrosome linker. The rootletin/CEP68 filaments are repetitive and highly ordered. Staggered rootletin molecules (N-to-N and C-to-C) within the filaments are 75 nm apart. Rootletin binds CEP68 via its C-terminal spectrin repeat-containing region in 75-nm intervals. The N-to-C distance of two rootletin molecules is ∼35 to 40 nm, leading to an estimated minimal rootletin length of ∼110 nm. CEP68 is important in forming rootletin filaments that branch off centrioles and to modulate the thickness of rootletin fibers. Thus, the centrosome linker consists of a vast network of repeating rootletin units with C-Nap1 as ring organizer and CEP68 as filament modulator.

Details

show
hide
Language(s): eng - English
 Dates: 2018-02-20
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1716840115
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 115 (10) Sequence Number: - Start / End Page: E2246 - E2253 Identifier: -