English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Cryo-EM in drug discovery: Achievements, limitations and prospects.

Renaud, J. P., Chari, A., Ciferri, C., Liu, W., Rémigy, H. W., Stark, H., et al. (2018). Cryo-EM in drug discovery: Achievements, limitations and prospects. Nature Reviews Drug Discovery, 17(7), 471-492. doi:10.1038/nrd.2018.77.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0001-7421-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-AF99-C
Genre: Journal Article

Files

show Files
hide Files
:
2599174.pdf (Publisher version), 1015KB
 
File Permalink:
-
Name:
2599174.pdf
Description:
-
Visibility:
Restricted ( Max Planck Society (every institute); )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Renaud, J. P., Author
Chari, A.1, Author              
Ciferri, C., Author
Liu, W., Author
Rémigy, H. W., Author
Stark, H.1, Author              
Wiesmann, C., Author
Affiliations:
1Department of Structural Dynamics, MPI for Biophysical Chemistry, Max Planck Society, ou_2205645              

Content

show
hide
Free keywords: -
 Abstract: Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical technique that can be used to determine the structure of biological macromolecules and assemblies. Historically, its potential for application in drug discovery has been heavily limited by two issues: the minimum size of the structures it can be used to study and the resolution of the images. However, recent technological advances - including the development of direct electron detectors and more effective computational image analysis techniques - are revolutionizing the utility of cryo-EM, leading to a burst of high-resolution structures of large macromolecular assemblies. These advances have raised hopes that single-particle cryo-EM might soon become an important tool for drug discovery, particularly if they could enable structural determination for 'intractable' targets that are still not accessible to X-ray crystallographic analysis. This article describes the recent advances in the field and critically assesses their relevance for drug discovery as well as discussing at what stages of the drug discovery pipeline cryo-EM can be useful today and what to expect in the near future.

Details

show
hide
Language(s): eng - English
 Dates: 2018-06-082018-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nrd.2018.77
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Reviews Drug Discovery
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 17 (7) Sequence Number: - Start / End Page: 471 - 492 Identifier: -