Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Collision Cross Sections and Ion Mobility Separation of Fragment Ions from Complex N-Glycans

Harvey, D. J., Watanabe, Y., Allen, J. D., Rudd, P., Pagel, K., Crispin, M., et al. (2018). Collision Cross Sections and Ion Mobility Separation of Fragment Ions from Complex N-Glycans. Journal of the American Society for Mass Spectrometry. doi:10.1007/s13361-018-1930-1.

Item is

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: http://hdl.handle.net/21.11116/0000-0001-758A-0 Versions-Permalink: http://hdl.handle.net/21.11116/0000-0001-758B-F
Genre: Zeitschriftenartikel

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Harvey, David J.1, 2, Autor
Watanabe, Yasunori2, 3, 4, Autor
Allen, Joel D.2, Autor
Rudd, Pauline5, Autor
Pagel, Kevin6, 7, Autor              
Crispin, Max2, Autor
Struwe, Weston B.3, 8, Autor
Affiliations:
1Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford, OX3 7FZ, UK, ou_persistent22              
2Biological Sciences and the Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, UK, ou_persistent22              
3Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK, ou_persistent22              
4Division of Structural Biology, University of Oxford, Wellcome Centre for Human Genetics, Roosevelt Drive, Oxford, OX3 7BN, UK, ou_persistent22              
5NIBRT GlycoScience Group, The National Institute for Bioprocessing Research and Training, Dublin, Ireland, ou_persistent22              
6Molecular Physics, Fritz Haber Institute, Max Planck Society, ou_634545              
7Institut für Chemie und Biochemie, Freien Universität BerlinTakustrasse 3, 14195, Berlin, Germany, ou_persistent22              
8Chemistry Research Laboratory, Department of Chemistry, University of Oxford, South Parks Road, Oxford, OX1 3TA, UK, ou_persistent22              

Inhalt

einblenden:
ausblenden:
Schlagwörter: Ion mobility, Complex N-glycans, Collision cross section, Glycomics
 Zusammenfassung: Ion mobility mass spectrometry (IM- MS) holds great potential for structural glycobiology, in particular in its ability to resolve glycan isomers. Generally, IM-MS has largely been applied to intact glycoconjugate ions with reports focusing on the separation of different adduct types. Here, we explore IM separation and report the collision cross section (CCS) of complex type N-glycans and their fragments in negative ion mode following collision-induced dis- sociation (CID). CCSs of isomeric fragment ions were found, in some cases, to reveal structural details that were not present in CID spectra themselves. Many fragment ions were confirmed as possessing multiple structure, details of which could be obtained by comparing their drift time profiles to different glycans. By using fragmen- tation both before and after mobility separation, information was gathered on the fragmentation pathways producing some of the ions. These results help demonstrate the utility of IM and will contribute to the growing use of IM-MS for glycomics.

Details

einblenden:
ausblenden:
Sprache(n): eng - Englisch
 Datum: 2017-12-152018-02-272018-04-19
 Publikationsstatus: Online publiziert
 Seiten: 12
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1007/s13361-018-1930-1
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Journal of the American Society for Mass Spectrometry
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: New York, NY : Springer
Seiten: - Band / Heft: - Artikelnummer: - Start- / Endseite: - Identifikator: ISSN: 1044-0305
CoNE: /journals/resource/954925590403