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  Dual-frequency irradiation CEST-MRI of endogenous bulk mobile proteins

Goerke, C., Breitling, J., Zaiss, M., Windschuh, J., Kunz, P., Schuenke, P., et al. (2018). Dual-frequency irradiation CEST-MRI of endogenous bulk mobile proteins. NMR in Biomedicine, 31(6), 1-14. doi:10.1002/nbm.3920.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-7C9F-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-806A-7
Genre: Journal Article

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Goerke, C, Author
Breitling, J, Author
Zaiss, M1, 2, Author              
Windschuh, J, Author
Kunz, P, Author
Schuenke, P, Author
Paech, D, Author
Longo, DL, Author
Klika, KD, Author
Ladd, ME, Author
Bachert, P, Author
Affiliations:
1Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497796              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              

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 Abstract: A novel MRI contrast is proposed which enables the selective detection of endogenous bulk mobile proteins in vivo. Such a non‐invasive imaging technique may be of particular interest for many diseases associated with pathological alterations of protein expression, such as cancer and neurodegenerative disorders. Specificity to mobile proteins was achieved by the selective measurement of intramolecular spin diffusion and the removal of semi‐solid macromolecular signal components by a correction procedure. For this purpose, the approach of chemical exchange saturation transfer (CEST) was extended to a radiofrequency (RF) irradiation scheme at two different frequency offsets (dualCEST). Using protein model solutions, it was demonstrated that the dualCEST technique allows the calculation of an image contrast which is exclusively sensitive to changes in concentration, molecular size and the folding state of mobile proteins. With respect to application in humans, dualCEST overcomes the selectivity limitations at relatively low magnetic field strengths, and thus enables examinations on clinical MR scanners. The feasibility of dualCEST examinations in humans was verified by a proof‐of‐principle examination of a brain tumor patient at 3 T. With its specificity for the mobile fraction of the proteome, its comparable sensitivity to conventional water proton MRI and its applicability to clinical MR scanners, this technique represents a further step towards the non‐invasive imaging of proteomic changes in humans.

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 Dates: 2018-06
 Publication Status: Published in print
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 Identifiers: DOI: 10.1002/nbm.3920
BibTex Citekey: CJMJPPDDKMP2018
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Title: NMR in Biomedicine
Source Genre: Journal
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Pages: - Volume / Issue: 31 (6) Sequence Number: - Start / End Page: 1 - 14 Identifier: eDoc: e3920