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  High frequency neural spiking and auditory signaling by ultrafast red-shifted optogenetics

Mager, T., Lopez de la Morena, D., Senn, V., Schlotte, J., D´Errico, A., Feldbauer, K., et al. (2018). High frequency neural spiking and auditory signaling by ultrafast red-shifted optogenetics. Nature Communications, 9: 1750. doi:10.1038/s41467-018-04146-3.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-6668-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-669C-C
Genre: Journal Article

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 Creators:
Mager, Thomas1, Author              
Lopez de la Morena, David2, 3, Author
Senn, Verena4, 5, Author              
Schlotte, Johannes1, Author              
D´Errico, Anna1, Author              
Feldbauer, Katrin1, Author              
Wrobel, Christian2, Author
Jung, Sangyong2, Author
Bodensiek, Kai2, Author
Rankovic, Vladan2, 6, Author
Browne, Lorcan2, 6, Author
Huet, Antoine2, 6, Author
Jüttner, Josephine7, Author
Wood, Phillip G.1, Author              
Letzkus, Johannes5, Author              
Moser, Tobias2, 3, 6, Author
Bamberg, Ernst1, Author              
Affiliations:
1Emeritusgruppe Biophysikalische Chemie, Max Planck Institute of Biophysics, Max Planck Society, ou_2253652              
2Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, D-37075, Göttingen, German, ou_persistent22              
3Göttingen Graduate School for Neuroscience and Molecular Biosciences, University of Göttingen, D-37075, Göttingen, Germany, ou_persistent22              
4Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, Max Planck Society, ou_2074314              
5Neocortical circuits Group, Max Planck Institute for Brain Research, Max Planck Society, ou_2461700              
6Auditory Neuroscience and Optogenetics Group, German Primate Center, D-37075, Göttingen, Germany, ou_persistent22              
7Friedrich Miescher Institute for Biomedical Research, CH-4058, Basel, Switzerland, ou_persistent22              

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Free keywords: Cochlea; Ion transport; Optogenetics
 Abstract: Optogenetics revolutionizes basic research in neuroscience and cell biology and bears potential for medical applications. We develop mutants leading to a unifying concept for the construction of various channelrhodopsins with fast closing kinetics. Due to different absorption maxima these channelrhodopsins allow fast neural photoactivation over the whole range of the visible spectrum. We focus our functional analysis on the fast-switching, red light-activated Chrimson variants, because red light has lower light scattering and marginal phototoxicity in tissues. We show paradigmatically for neurons of the cerebral cortex and the auditory nerve that the fast Chrimson mutants enable neural stimulation with firing frequencies of several hundred Hz. They drive spiking at high rates and temporal fidelity with low thresholds for stimulus intensity and duration. Optical cochlear implants restore auditory nerve activity in deaf mice. This demonstrates that the mutants facilitate neuroscience research and future medical applications such as hearing restoration.

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Language(s): eng - English
 Dates: 2017-05-182018-04-062018-05-01
 Publication Status: Published online
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-018-04146-3
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 9 Sequence Number: 1750 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723