English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Similar but not the same: first kinetic and structural analyses of a methanol dehydrogenase containing a europium ion in the active site

Jahn, B., Pol, A., Lumpe, H., Barends, T., Dietl, A., Hogendoorn, C., et al. (2018). Similar but not the same: first kinetic and structural analyses of a methanol dehydrogenase containing a europium ion in the active site. ChemBioChem: A European Journal of Chemical Biology, 19(11), 1147-1153. doi:10.1002/cbic.201800130.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0001-8E3A-F Version Permalink: http://hdl.handle.net/21.11116/0000-0003-CF9B-6
Genre: Journal Article

Files

show Files
hide Files
:
ChemBioChem_19_2018_1147.pdf (Any fulltext), 651KB
 
File Permalink:
-
Name:
ChemBioChem_19_2018_1147.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, Heidelberg; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Locator:
https://doi.org/10.1002/cbic.201800130 (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Jahn, Bérénice, Author
Pol, Arjan, Author
Lumpe, Henning, Author
Barends, Thomas1, Author              
Dietl, Andreas1, Author              
Hogendoorn, Carmen, Author
den Camp, Huub J. M. Op, Author
Daumann, Lena J., Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Content

show
hide
Free keywords: -
 Abstract: Since the discovery of the biological relevance of rare earth elements (REEs) for numerous different bacteria, questions concerning the advantages of REEs in the active sites of methanol dehydrogenases (MDHs) over calcium(II) and of why bacteria prefer light REEs have been a subject of debate. Here we report the cultivation and purification of the strictly REE-dependent methanotrophic bacterium Methylacidiphilum fumariolicum SolV with europium(III), as well as structural and kinetic analyses of the first methanol dehydrogenase incorporating Eu in the active site. Crystal structure determination of the Eu-MDH demonstrated that overall no major structural changes were induced by conversion to this REE. Circular dichroism (CD) measurements were used to determine optimal conditions for kinetic assays, whereas inductively coupled plasma mass spectrometry (ICP-MS) showed 70 % incorporation of Eu in the enzyme. Our studies explain why bacterial growth of SolV in the presence of Eu3+ is significantly slower than in the presence of La3+ /Ce3+ /Pr3+ : Eu-MDH possesses a decreased catalytic efficiency. Although REEs have similar properties, the differences in ionic radii and coordination numbers across the series significantly impact MDH efficiency.

Details

show
hide
Language(s): eng - English
 Dates: 2018-03-092018-05-102018-04-162018-06-04
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ChemBioChem: A European Journal of Chemical Biology
  Other : ChemBioChem
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim, Germany : Wiley-VCH
Pages: - Volume / Issue: 19 (11) Sequence Number: - Start / End Page: 1147 - 1153 Identifier: ISSN: 1439-4227
CoNE: /journals/resource/110978984568897_1