English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Kinetics of the B-A transition of DNA: Analysis of potential contributions to a reaction barrier.

Porschke, D. (2018). Kinetics of the B-A transition of DNA: Analysis of potential contributions to a reaction barrier. European Biophysics Journal, 47(4), 325-332. doi:10.1007/s00249-018-1276-4.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0001-9386-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-0172-C
Genre: Journal Article

Files

show Files
hide Files
:
2607970.pdf (Publisher version), 867KB
 
File Permalink:
-
Name:
2607970.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Porschke, D.1, Author              
Affiliations:
1Research Group of Biomolecular Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578610              

Content

show
hide
Free keywords: B-A transition; Kinetics; Magic angle measurements; Electrostatics; Solvent isotope effect
 Abstract: Because of open problems in the relation between results obtained by relaxation experiments and molecular dynamics simulations on the B-A transition of DNA, relaxation measurements of the B-A dynamics have been extended to a wider range of conditions. Field-induced reaction effects are measured selectively by the magic angle technique using a novel cell construction preventing perturbations from cell window anisotropy. The kinetics was recorded for the case of poly[d(AT)] up to the salt concentration limit of 4.4 mM, where aggregation does not yet interfere. Now experimental data on the B-A dynamics are available for poly[d(AT)] at salt concentrations of 0.18, 0.73, 2.44 and 4.4 mM. In all cases, a spectrum of time constants is found, ranging from ~ 10 μs up to components approaching ~ 1 ms. The relatively small dependence of these data on the salt concentration indicates that electrostatic effects on the kinetics are not as strong as may be expected. The ethanol content at the transition center is a linear function of the logarithm of the salt concentration, and the slope is close to that expected from polyelectrolyte theory. The B-A transition dynamics was also measured in D2O at a salt concentration of 2.4 mM: the center of the transition is found at 20.0 mol/l H2O and at 20.1 mol/l D2O with an estimated accuracy of ± 0.1 mol/l; the spectrum of time constants at the respective transition centers is very similar. The experimental results are discussed regarding the data obtained by molecular dynamics simulations.

Details

show
hide
Language(s): eng - English
 Dates: 2018-02-052018-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s00249-018-1276-4
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: European Biophysics Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 47 (4) Sequence Number: - Start / End Page: 325 - 332 Identifier: -