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  Tissue embedding in a silica hydrogel for functional investigations

Miazzi, F., Kaltofen, S., Bello, J., Hansson, B. S., & Wicher, D. (submitted). Tissue embedding in a silica hydrogel for functional investigations.

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http://dx.doi.org/10.1101/357962 (Preprint)
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 Creators:
Miazzi, Fabio1, 2, Author           
Kaltofen, Sabine1, Author           
Bello, Jan1, Author           
Hansson, Bill S.1, Author           
Wicher, Dieter1, Author           
Affiliations:
1Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421894              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

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 Abstract: Embedding procedures can be challenging for delicate specimens. We present a method based on a sodium metasilicate (waterglass) gel to embed tissue samples for acute physiological studies. We show that the application of such a colloidal gel has minimal effects on the properties of buffered solutions and cell activity, allowing functional investigations on sensitive cells such as ciliated insect olfactory neurons.

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 Dates: 2018-06-28
 Publication Status: Submitted
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 Rev. Type: No review
 Identifiers: Other: HAN311
DOI: 10.1101/357962
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Title: bioRxiv
Source Genre: Journal
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