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  A microscopy-based approach for studying meiosis in live and fixed human oocytes.

Zielinska, A., & Schuh, M. (2018). A microscopy-based approach for studying meiosis in live and fixed human oocytes. In H. Maiato (Ed.), Methods in Cell Biology (pp. 315-333). Amsterdam: Elsevier. doi:10.1016/bs.mcb.2018.03.039.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-98C5-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-C25B-C
Genre: Book Chapter

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2609105.pdf (Publisher version), 864KB
 
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 Creators:
Zielinska, A.1, Author              
Schuh, M.1, Author              
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

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Free keywords: Meiosis; Human oocyte; Microscopy; Live cell; Immunofluorescence; Aneuploidy; Microtubules; Kinetochores
 Abstract: Human eggs frequently carry an incorrect number of chromosomes, which is a leading cause of pregnancy loss and congenital disorders. The origins of high aneuploidy rates in human eggs have remained largely unclear. This is due to two main reasons: first, the availability of human eggs is limited so that studies of fixed human eggs typically involve very small numbers and limited quantifications. Second, methods for studying meiosis in live human eggs have been missing. The ever rising prevalence of Assisted Reproductive Technologies has facilitated a recent breakthrough in the field. The mechanistic basis of meiosis in humans can now be examined directly in live eggs. Here, we present a robust method for culturing human eggs in vitro and describe how meiotic processes in human eggs can be studied in real time using fluorescent reporters. We further describe methods for the in-depth analysis of immunolabeled eggs by super-resolution light microscopy.

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Language(s): eng - English
 Dates: 2018-05-262018
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/bs.mcb.2018.03.039
 Degree: -

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Title: Methods in Cell Biology
Source Genre: Series
 Creator(s):
Maiato, H., Editor
Affiliations:
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 145 Sequence Number: - Start / End Page: 315 - 333 Identifier: -