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  Reduced dyes enhance single-molecule localization density for live superresolution imaging.

Carlini, L., Benke, A., Reymond, L., Lukinavicius, G., & Manley, S. (2014). Reduced dyes enhance single-molecule localization density for live superresolution imaging. ChemPhysChem, 15(4), 750-755. doi:10.1002/cphc.201301004.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-A61C-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-A61F-2
Genre: Journal Article

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2616409.pdf (Publisher version), 2MB
 
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 Creators:
Carlini, L., Author
Benke, A., Author
Reymond, L., Author
Lukinavicius, G.1, Author              
Manley, S., Author
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              

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Free keywords: fluorophores; live-cell imaging; rhodamine; single-molecule studies; superresolution microscopy
 Abstract: Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can result in up to ten-fold higher densities of single molecule localizations, and more photons per localization as compared with unmodified dyes. These two parameters directly impact the achievable resolution, and we see a significant improvement in the quality of live-cell point-localization super-resolution images taken with reduced dyes. These improvements carry over to increase the density of trajectories for single-molecule tracking experiments.

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Language(s): eng - English
 Dates: 2014-02-19
 Publication Status: Published online
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/cphc.201301004
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Title: ChemPhysChem
Source Genre: Journal
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Pages: - Volume / Issue: 15 (4) Sequence Number: - Start / End Page: 750 - 755 Identifier: -