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  Acoustic plate mode sensor for immunochemical reactions

Dahint, R., Grunze, M., Josse, F., & Renken, J. (1994). Acoustic plate mode sensor for immunochemical reactions. Analytical Chemistry, 66(18), 2888-2892. doi:10.1021/ac00090a015.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0001-A70B-7 Version Permalink: https://hdl.handle.net/21.11116/0000-0001-A720-E
Genre: Journal Article

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AnalytChem_66_1994_2888.pdf (Any fulltext), 533KB
 
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https://pubs.acs.org/doi/pdf/10.1021/ac00090a015 (Any fulltext)
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 Creators:
Dahint, R., Author
Grunze, Michael1, Author           
Josse, F., Author
Renken, J., Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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 Abstract: A practical immunosensor, based on a 150-MHz acoustic plate mode (APM) device on ZX-LiNbOa has been built and is employed to monitor directly, in real time, antigen-antibody reactions via mass loading of the crystal surface. The results show frequency shifts ~ 1 kHz above the noise level upon antigen adsorption. The mass density detection limit of our present sensor is ~200 pg/mm2, corresponding to 8.10s molecules/ mm2 utilizing a typical IgG of 150 000 Da. Antigen binding capacities of aminosilane films are measured independently by a modified ELISA test and are related to the APM sensor response.

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Language(s): eng - English
 Dates: 1994-02-241994-05-171994-09-01
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/ac00090a015
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Title: Analytical Chemistry
  Abbreviation : Anal. Chem.
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 66 (18) Sequence Number: - Start / End Page: 2888 - 2892 Identifier: ISSN: 0003-2700
CoNE: https://pure.mpg.de/cone/journals/resource/111032812862552