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  Soft Picosecond Infrared Laser Extraction of Highly Charged Proteins and Peptides from Bulk Liquid Water for Mass Spectrometry

Lu, Y., Pieterse, C. L., Robertson, W., & Miller, R. J. D. (2018). Soft Picosecond Infrared Laser Extraction of Highly Charged Proteins and Peptides from Bulk Liquid Water for Mass Spectrometry. Analytical Chemistry, 90(7), 4422-4428. doi:10.1021/acs.analchem.7b04306.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-AB67-B Version Permalink: http://hdl.handle.net/21.11116/0000-0001-AB68-A
Genre: Journal Article

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acs.analchem.7b04306.pdf (Publisher version), 2MB
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acs.analchem.7b04306.pdf
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ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
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2018
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© American Chemical Society

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 Creators:
Lu, Y.1, Author              
Pieterse, C. L.1, Author              
Robertson, W.1, Author              
Miller, R. J. D.1, Author              
Affiliations:
1Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              

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 Abstract: We report the soft laser extraction and production of highly charged peptide and protein ions for mass spectrometry directly from bulk liquid water at atmospheric pressure and room temperature, using picosecond infrared laser ablation. Stable ion signal from singly charged small molecules, as well as highly charged biomolecular ions, from aqueous solutions at low laser pulse fluence (∼0.3 J cm–2) is demonstrated. Sampling via single picosecond laser pulses is shown to extract less than 27 pL of volume from the sample, producing highly charged peptide and protein ions for mass spectrometry detection. The ablation and ion generation is demonstrated to be soft in nature, producing natively folded proteins ions under sample conditions described for native mass spectrometry. The method provides laser-based sampling flexibility, precision and control with highly charged ion production directly from water at low and near neutral pH. This approach does not require an additional ionization device or high voltage applied directly to the sample.

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Language(s): eng - English
 Dates: 2017-10-182018-03-092018-03-092018-04-03
 Publication Status: Published in print
 Pages: 7
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1021/acs.analchem.7b04306
 Degree: -

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Project name : We would like to thank Djordje Gitaric and Josef Gonschior for their valuable design and engineering contributions, as well as Erik Friedling for designing the capillary extension. We would also like to thank Jean-Michel Boudreau for his e ff orts in native bu ff er preparation and Maria Grigera for her graphical contributions. This work was supported by the Max Planck Institute.
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Title: Analytical Chemistry
  Abbreviation : Anal. Chem.
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 90 (7) Sequence Number: - Start / End Page: 4422 - 4428 Identifier: ISSN: 0003-2700
CoNE: /journals/resource/111032812862552